The DAPI was excited by the 355nm laser and its emission collected using a 450/50 filter. Preparation and sequencing of RNA-seq libraries RNA prepared from flow sorted cells was quality controlled on a Bioanalyzer 2100 (Agilent) using RNA Pico 6000 chips (Agilent 5067C1513). for 3 minutes at 4C before RNA extraction with TRI Reagent. 20% … Continue reading The DAPI was excited by the 355nm laser and its emission collected using a 450/50 filter